1A0L

HUMAN BETA-TRYPTASE: A RING-LIKE TETRAMER WITH ACTIVE SITES FACING A CENTRAL PORE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1997-02
Detector	MARRESEARCH
Spacegroup name	P 41
Unit cell lengths	82.930, 82.930, 172.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.197
Rwork	0.197
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	24.690 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.000
Rmerge	0.121
Number of reflections	23164 *
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.1 *	4 *	pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium chloride	1.7 (M)
2	1	reservoir	ammonium sulfate	3 (M)
3	1	reservoir	3-(N-morpholino)propanesulphonic acid	0.2 (M)