1A0K

PROFILIN I FROM ARABIDOPSIS THALIANA

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	43.050, 43.530, 59.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.172
Rwork	0.172
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nul
RMSD bond length	0.014
RMSD bond angle	23.650 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.066	0.120
Total number of observations	48373 *
Number of reflections	5530
<I/σ(I)>	0.2176	10.13
Completeness [%]	90.4	55.5
Redundancy	2.6	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		2.0 M AMMONIUM SULFATE, 0.1 M CITRATE PH 5.0, 10 MM DTT, 0.2 MM EDTA

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0 (M)
2	1	reservoir	citrate	0.1 (M)
3	1	reservoir	DTT	10 (mM)
4	1	reservoir	EDTA	0.2 (mM)