1A09
C-src (SH2 domain) complexed with ace-formyl phosphotyr-glu-(n,n-dipentyl amine)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.900, 66.000, 74.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.650 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.162 |
| Number of reflections | 17257 | |
| <I/σ(I)> | 13.3 | 4.4 |
| Completeness [%] | 92.0 | 93.6 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | PROTEIN WAS CRYSTALLIZED FROM 0.1 M ACETATE, PH 4.6, 2 M NAFORMATE AT 4 C. THE CRYSTAL WAS SOAKED IN 10% PEG400, 10% GLYCEROL PRIOR TO DATA COLLECTION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 125 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 350 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | EDTA | 5 (mM) | |
| 6 | 1 | reservoir | acetate | 0.1 (M) | |
| 7 | 1 | reservoir | sodium formate | 2 (M) |
