19GS
Glutathione s-transferase p1-1
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-26 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.742, 89.984, 68.842 |
Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gss |
RMSD bond length | 0.008 |
RMSD bond angle | 22.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.273 |
Total number of observations | 84660 * | |
Number of reflections | 35002 | |
<I/σ(I)> | 14.3 | 3.5 |
Completeness [%] | 93.3 | 87 * |
Redundancy | 2.42 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 22 * | Oakley, A.J., (1997) J.Mol.Biol., 274, 84. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | phosphate | 10 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | mercaptoethanol | 2 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 20-34 (%(w/v)) | |
6 | 1 | reservoir | dithiothreitol | 50-80 (mM) | |
7 | 1 | reservoir | MES | 100 (mM) |