195D
X-RAY STRUCTURES OF THE B-DNA DODECAMER D(CGCGTTAACGCG) WITH AN INVERTED CENTRAL TETRANUCLEOTIDE AND ITS NETROPSIN COMPLEX
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 258 |
Detector technology | AREA DETECTOR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.490, 40.870, 67.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.300 |
R-factor | 0.162 |
Rwork | 0.162 |
RMSD bond length | 0.015 |
RMSD bond angle | 3.600 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.300 |
Rmerge | 0.051 * |
Number of reflections | 2725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 282 | pH 7.50, VAPOR DIFFUSION, temperature 282.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE_HCL | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 1 equivalent of | |
2 | 1 | drop | 5 equivalent of | ||
3 | 1 | drop | spermine tetrachloride | 1.8 equivalent of | |
4 | 1 | drop | netropsin | 1.2 equivalent of | |
5 | 1 | drop | MPD | 30 (%) | |
6 | 1 | reservoir | 40 (%) |