192D
RECOMBINATION-LIKE STRUCTURE OF D(CCGCGG)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Detector technology | DIFFRACTOMETER |
| Detector | OTHER |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.330, 44.040, 38.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.920 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.280 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.920 |
| Rmerge | 0.056 * |
| Total number of observations | 13022 * |
| Number of reflections | 2297 |
| Completeness [%] | 96.5 * |
| Redundancy | 5.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | pH 6.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | TETRA-ARGININE | ||
| 5 | 1 | 1 | NA CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hexanucleotide | 0.83 (mM) | |
| 2 | 1 | drop | spermine | 2.8 (mM) | |
| 3 | 1 | drop | tetra arginine | 0.6 (mM) | |
| 4 | 1 | drop | sodium ccacodylate | 18 (mM) | |
| 5 | 1 | drop | MPD | 20 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 45 (%) |
