191D
CRYSTAL STRUCTURE OF INTERCALATED FOUR-STRANDED D(C3T)
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 28.281, 44.341, 50.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.22500 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 4.000 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | pH 6.50, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | SRCL2 | ||
| 6 | 1 | 1 | SR CACODYLATE | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium salt | 2.3 (mM) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | spermine | 0.12 (mM) | |
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | strontium cacodylate | 40 (mM) | |
| 6 | 1 | drop | MPD | 10 (%) | |
| 7 | 1 | reservoir | MPD | 30 (%) |
