190L
A HELIX INITIATION SIGNAL IN T4 LYSOZYME IDENTIFIED BY POLYALANINE MUTAGENESIS
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.600, 60.600, 95.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.171 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.250 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.064 * |
| Completeness [%] | 88.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | A.E. Eriksson, (1993) J. Mol. Biol., 229, 747. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | phosphate | 2.0 (M) | |
| 4 | 1 | reservoir | beta-mercaptoethanol |
