184D
SELF-ASSOCIATION OF A DNA LOOP CREATES A QUADRUPLEX: CRYSTAL STRUCTURE OF D(GCATGCT) AT 1.8 ANGSTROMS RESOLUTION
Experimental procedure
Temperature [K] | 279 |
Detector technology | DIFFRACTOMETER |
Detector | SYNTEX P21 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 22.520, 59.370, 24.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 1.800 |
R-factor | 0.214 |
Rwork | 0.214 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.025 * |
Total number of observations | 4259 * |
Number of reflections | 1606 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 18-22 * | pH 6.80, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | HEXANE-1,6-DIOL | ||
3 | 1 | 1 | LI CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | SPERMINE_HCL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | HEXANE-1,6-DIOL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | heptanucleotide | 5 (mg/ml) | |
2 | 1 | drop | lithium cacodylate | 50 (mM) | |
3 | 1 | drop | magnesium chloride | 50 (mM) | |
4 | 1 | drop | spermine tetrahydrochloride | 3 (mM) | |
5 | 1 | drop | hexane-1,6-diol | 10 (%(v/v)) | |
6 | 1 | reservoir | hexane-1,6-diol | 50 (%(v/v)) |