178L
Protein flexibility and adaptability seen in 25 crystal forms of T4 LYSOZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 290 |
Detector technology | AREA DETECTOR |
Collection date | 1992-01-07 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 118.900, 118.900, 39.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.500 - 2.710 |
R-factor | 0.192 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.200 |
Data reduction software | Xuong-Hamlin |
Data scaling software | Rotovata/Agrovata |
Phasing software | MRX |
Refinement software | TNT |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.500 |
High resolution limit [Å] | 2.710 |
Number of reflections | 6596 |
Completeness [%] | 81.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 24 (%(w/v)) | |
2 | 1 | reservoir | 0.16 (M) | ||
3 | 1 | reservoir | sodium citrate | 0.08 (M) |