178L

Protein flexibility and adaptability seen in 25 crystal forms of T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1992-01-07
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 4 21 2
Unit cell lengths	118.900, 118.900, 39.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.710
R-factor	0.192
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.200
Data reduction software	Xuong-Hamlin
Data scaling software	Rotovata/Agrovata
Phasing software	MRX
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	19.500
High resolution limit [Å]	2.710
Number of reflections	6596
Completeness [%]	81.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	24 (%(w/v))
2	1	reservoir		0.16 (M)
3	1	reservoir	sodium citrate	0.08 (M)