16VP

CONSERVED CORE OF THE HERPES SIMPLEX VIRUS TRANSCRIPTIONAL REGULATORY PROTEIN VP16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	95
Detector technology	RAXIS AND MAR
Spacegroup name	P 21 21 21
Unit cell lengths	59.500, 77.100, 84.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.100
R-factor	0.192 *
Rwork	0.192
R-free	0.26000
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.068	0.356
Number of reflections	21163	1008 *
<I/σ(I)>	11.3	2.8
Completeness [%]	96.8	92.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4	16 *	pH 4.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%(w/v))
2	1	reservoir	potassium phosphate	50 (mM)
3	1	drop	protein	10 (mg/ml)