16GS
GLUTATHIONE S-TRANSFERASE P1-1 APO FORM 3
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-25 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.537, 89.797, 68.673 |
| Unit cell angles | 90.00, 97.79, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gss |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.910 |
| Rmerge | 0.053 | 0.329 |
| Total number of observations | 96823 * | |
| Number of reflections | 31323 * | |
| <I/σ(I)> | 17.7 | 2.6 |
| Completeness [%] | 95.1 | 91.5 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 22 * | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | phosphate | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 23 (%(w/v)) | |
| 6 | 1 | reservoir | dithiothreitol | 55 (mM) | |
| 7 | 1 | reservoir | MES | 100 (mM) |
