154D
DNA DISTORTION IN BIS-INTERCALATED COMPLEXES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 27.090, 27.090, 81.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.100 - 2.500 |
| R-factor | 0.239 |
| RMSD bond length | 0.021 |
| Refinement software | PROLSQ ((MODIFIED BY G.J.QUIGLEY)) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.066 * |
| Number of reflections | 1183 |
| Completeness [%] | 98.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 18 (mM) | |
| 2 | 1 | drop | DNA | 0.7 (mM) | single strand concentration |
| 3 | 1 | drop | Flexi Di | 0.2 (mM) | |
| 4 | 1 | drop | 3.9 (mM) | ||
| 5 | 1 | drop | MPD | 3.1 (%) | |
| 6 | 1 | reservoir | 10 (%) |
