14ZU

Crystal Structure of FtsZ with Z17497990

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.461, 88.461, 179.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.270 - 1.793
Rwork	0.178
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.795
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.230	1.824
High resolution limit [Å]	1.793	1.793
Rmerge	0.171	2.474
Rmeas	0.178	2.567
Rpim	0.048	0.681
Total number of observations	993234	50588
Number of reflections	74231	3720
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.999	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-926	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.