14ZA

Crystal Structure of FtsZ with Z734736324

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.025, 88.025, 177.424
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.051 - 2.826
Rwork	0.159
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.622
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.013	2.875
High resolution limit [Å]	2.826	2.826
Rmerge	0.457	2.532
Rmeas	0.475	2.630
Rpim	0.131	0.708
Total number of observations	239859	12780
Number of reflections	18420	928
<I/σ(I)>	5.9
Completeness [%]	98.9
Redundancy	13	13.8
CC(1/2)	0.980	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-735	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.