14YZ

Crystal Structure of FtsZ with Z1639162606

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.001, 88.001, 177.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.039 - 2.546
Rwork	0.167
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.646
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.590
High resolution limit [Å]	2.546	2.546
Rmerge	0.336	2.277
Rmeas	0.350	2.364
Rpim	0.095	0.634
Total number of observations	343688	18128
Number of reflections	25392	1300
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	13.5	13.9
CC(1/2)	0.993	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-727	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.