14YS

Crystal Structure of FtsZ with Z19735192

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	87.681, 87.681, 175.553
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.879 - 2.565
Rwork	0.165
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.503
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.840	2.609
High resolution limit [Å]	2.565	2.565
Rmerge	0.328	2.474
Rmeas	0.342	2.570
Rpim	0.094	0.690
Total number of observations	312470	17006
Number of reflections	24042	1220
<I/σ(I)>	7.1
Completeness [%]	98.4
Redundancy	13	13.9
CC(1/2)	0.991	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-690	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.