14YO

Crystal Structure of FtsZ with Z69092635

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.436, 88.436, 178.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.257 - 1.917
Rwork	0.181
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.721
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.218	1.950
High resolution limit [Å]	1.917	1.917
Rmerge	0.221	2.753
Rmeas	0.229	2.862
Rpim	0.063	0.778
Total number of observations	807987	40281
Number of reflections	60539	2998
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.997	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-657	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.