14YE

Crystal Structure of FtsZ with Z1217960891

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.629, 88.629, 179.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.354 - 1.867
Rwork	0.180
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.795
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.314	1.899
High resolution limit [Å]	1.867	1.867
Rmerge	0.222	2.833
Rmeas	0.231	2.944
Rpim	0.063	0.799
Total number of observations	880095	44160
Number of reflections	65821	3262
<I/σ(I)>	9.1
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.997	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-633	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.