14YD

Crystal Structure of FtsZ with Z90503851

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.939, 88.939, 180.142
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.211 - 1.980
Rwork	0.181
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.753
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.173	2.014
High resolution limit [Å]	1.980	1.980
Rmerge	0.196	2.998
Rmeas	0.204	3.118
Rpim	0.056	0.854
Total number of observations	746288	36898
Number of reflections	55923	2774
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	13.3	13.3
CC(1/2)	0.998	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-624	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.