14XI

Crystal Structure of FtsZ with Z57472297

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.331, 88.331, 178.439
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.205 - 2.098
Rwork	0.171
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.801
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.166	2.134
High resolution limit [Å]	2.098	2.098
Rmerge	0.279	2.836
Rmeas	0.291	2.967
Rpim	0.082	0.870
Total number of observations	582183	26290
Number of reflections	45935	2267
<I/σ(I)>	8
Completeness [%]	100.0
Redundancy	12.7	11.6
CC(1/2)	0.996	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-511	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.