14XE

Crystal Structure of FtsZ with F9995-0998

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	89.013, 89.013, 179.717
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.239 - 1.914
Rwork	0.186
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.767
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.201	1.947
High resolution limit [Å]	1.914	1.914
Rmerge	0.154	2.123
Rmeas	0.161	2.207
Rpim	0.044	0.600
Total number of observations	825870	41846
Number of reflections	61885	3113
<I/σ(I)>	12.4
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.999	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-49	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.