14WZ

Crystal Structure of FtsZ with Z192955056

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.688, 88.688, 179.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.383 - 1.921
Rwork	0.181
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.732
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.344	1.954
High resolution limit [Å]	1.921	1.921
Rmerge	0.186	2.525
Rmeas	0.193	2.625
Rpim	0.053	0.716
Total number of observations	808766	40259
Number of reflections	60641	3002
<I/σ(I)>	10.9
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-470	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.