14WX

Crystal Structure of FtsZ with Z906021418

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.603, 88.603, 178.904
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.341 - 1.861
Rwork	0.181
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.758
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.301	1.893
High resolution limit [Å]	1.861	1.861
Rmerge	0.166	2.283
Rmeas	0.172	2.372
Rpim	0.047	0.639
Total number of observations	885361	44890
Number of reflections	66419	3331
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	13.3	13.5
CC(1/2)	0.999	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-467	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.