14WT

Crystal Structure of FtsZ with Z1295863442

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.510, 88.510, 179.318
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.294 - 2.131
Rwork	0.181
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.680
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.255	2.167
High resolution limit [Å]	2.131	2.131
Rmerge	0.347	2.903
Rmeas	0.361	3.021
Rpim	0.098	0.834
Total number of observations	596793	29286
Number of reflections	44309	2234
<I/σ(I)>	7
Completeness [%]	100.0
Redundancy	13.5	13.1
CC(1/2)	0.993	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-461	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.