14WS

Crystal Structure of FtsZ with Z2234920345

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.445, 88.445, 178.827
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.966 - 2.099
Rwork	0.183
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.743
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.929	2.136
High resolution limit [Å]	2.099	2.099
Rmerge	0.293	2.453
Rmeas	0.305	2.560
Rpim	0.084	0.731
Total number of observations	617080	27847
Number of reflections	46082	2270
<I/σ(I)>	5.8
Completeness [%]	100.0
Redundancy	13.4	12.3
CC(1/2)	0.995	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-459	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.