14WL

Crystal Structure of FtsZ with Z52425517

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.511, 88.511, 178.412
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.295 - 1.897
Rwork	0.182
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.707
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.256	1.930
High resolution limit [Å]	1.897	1.897
Rmerge	0.192	2.665
Rmeas	0.199	2.769
Rpim	0.054	0.746
Total number of observations	832860	41603
Number of reflections	62378	3094
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	13.4	13.4
CC(1/2)	0.998	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-425	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.