14WF

Crystal Structure of FtsZ with Z26781952

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.784, 88.784, 180.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.149 - 2.089
Rwork	0.178
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.703
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.112	2.125
High resolution limit [Å]	2.089	2.089
Rmerge	0.194	2.334
Rmeas	0.202	2.438
Rpim	0.055	0.700
Total number of observations	639431	29239
Number of reflections	47714	2401
<I/σ(I)>	9.8
Completeness [%]	100.0
Redundancy	13.4	12.2
CC(1/2)	0.998	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-417	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.