14VY

Crystal Structure of FtsZ with Z1983897532

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	89.026, 89.026, 178.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.227 - 2.041
Rwork	0.203
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.841
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.190	2.068
High resolution limit [Å]	2.033	2.033
Rmerge	0.312	3.400
Rmeas	0.326	3.539
Rpim	0.090	0.980
Total number of observations	664783	32571
Number of reflections	51039	2549
<I/σ(I)>	7.6
Completeness [%]	99.5
Redundancy	13	12.8
CC(1/2)	0.985	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-395	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.