14VV

Crystal Structure of FtsZ with Z56618096

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.207, 88.207, 176.593
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.142 - 2.101
Rwork	0.175
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.588
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.104	2.137
High resolution limit [Å]	2.101	2.101
Rmerge	0.210	2.118
Rmeas	0.218	2.211
Rpim	0.060	0.631
Total number of observations	578408	27569
Number of reflections	44371	2250
<I/σ(I)>	9.8
Completeness [%]	98.3
Redundancy	13	12.3
CC(1/2)	0.998	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-357	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.