14VT

Crystal Structure of FtsZ with Z68639747

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.006, 88.006, 176.868
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.042 - 2.084
Rwork	0.172
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.690
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.003	2.120
High resolution limit [Å]	2.084	2.084
Rmerge	0.176	2.038
Rmeas	0.183	2.128
Rpim	0.050	0.611
Total number of observations	617189	27914
Number of reflections	46145	2296
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	13.4	12.2
CC(1/2)	0.998	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-343	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.