14VS

Crystal Structure of FtsZ with Z45497619

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	87.758, 87.758, 176.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.918 - 2.262
Rwork	0.167
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.776
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.879	2.301
High resolution limit [Å]	2.262	2.262
Rmerge	0.281	3.073
Rmeas	0.292	3.196
Rpim	0.079	0.872
Total number of observations	483306	24076
Number of reflections	35898	1798
<I/σ(I)>	8
Completeness [%]	100.0
Redundancy	13.5	13.4
CC(1/2)	0.996	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-342	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.