14VC

Crystal Structure of FtsZ with F5857-3414

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.631, 88.631, 178.847
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.052 - 2.439
Rwork	0.178
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.663
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.015	2.481
High resolution limit [Å]	2.439	2.439
Rmerge	0.457	2.678
Rmeas	0.476	2.784
Rpim	0.129	0.757
Total number of observations	401290	19782
Number of reflections	29563	1472
<I/σ(I)>	6.2
Completeness [%]	100.0
Redundancy	13.6	13.4
CC(1/2)	0.987	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-225	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.