14VB

Crystal Structure of FtsZ with F3375-2883

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	89.006, 89.006, 181.285
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.257 - 2.061
Rwork	0.187
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.583
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.220	2.097
High resolution limit [Å]	2.061	2.061
Rmerge	0.255	2.992
Rmeas	0.265	3.118
Rpim	0.073	0.875
Total number of observations	667625	31235
Number of reflections	49992	2475
<I/σ(I)>	7.9
Completeness [%]	100.0
Redundancy	13.4	12.6
CC(1/2)	0.997	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-223	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.