14UX

Crystal Structure of FtsZ with F2124-0068

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.645, 88.645, 179.732
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.070 - 2.051
Rwork	0.180
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.692
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.033	2.087
High resolution limit [Å]	2.052	2.052
Rmerge	0.234	2.416
Rmeas	0.243	2.517
Rpim	0.067	0.703
Total number of observations	665985	31779
Number of reflections	49876	2483
<I/σ(I)>	8.5
Completeness [%]	100.0
Redundancy	13.4	12.8
CC(1/2)	0.997	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-203	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.