14UV

Crystal Structure of FtsZ with F5856-0194

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.821, 88.821, 181.626
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.177 - 2.016
Rwork	0.192
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.664
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.140	2.051
High resolution limit [Å]	2.016	2.016
Rmerge	0.160	2.074
Rmeas	0.167	2.159
Rpim	0.046	0.596
Total number of observations	707982	34606
Number of reflections	53261	2639
<I/σ(I)>	12.5
Completeness [%]	100.0
Redundancy	13.3	13.1
CC(1/2)	0.998	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-186	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.