14UT

Crystal Structure of FtsZ with F1906-0090

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.671, 88.671, 180.323
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.090 - 2.268
Rwork	0.185
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.580
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.053	2.307
High resolution limit [Å]	2.268	2.268
Rmerge	0.310	2.267
Rmeas	0.322	2.358
Rpim	0.088	0.646
Total number of observations	497842	24358
Number of reflections	37098	1831
<I/σ(I)>	6.5
Completeness [%]	100.0
Redundancy	13.4	13.3
CC(1/2)	0.993	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-174	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.