14UQ

Crystal Structure of FtsZ with F6300-0026

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.735, 88.735, 179.639
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.110 - 2.147
Rwork	0.186
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.604
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.073	2.184
High resolution limit [Å]	2.147	2.147
Rmerge	0.301	2.425
Rmeas	0.313	2.520
Rpim	0.086	0.685
Total number of observations	585339	29360
Number of reflections	43604	2175
<I/σ(I)>	6.6
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.995	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-149	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.