14UP

Crystal Structure of FtsZ with F6616-2660

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.373, 88.373, 178.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.226 - 1.953
Rwork	0.179
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.735
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.187	1.986
High resolution limit [Å]	1.953	1.953
Rmerge	0.209	2.752
Rmeas	0.217	2.861
Rpim	0.059	0.780
Total number of observations	761671	38194
Number of reflections	57117	2854
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.997	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-146	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.