14UN

Crystal Structure of FtsZ with F1902-0062

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.688, 88.688, 178.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.383 - 1.928
Rwork	0.181
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.737
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.344	1.961
High resolution limit [Å]	1.928	1.928
Rmerge	0.198	2.499
Rmeas	0.206	2.598
Rpim	0.056	0.708
Total number of observations	798244	39411
Number of reflections	59771	2932
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	13.4	13.4
CC(1/2)	0.998	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-131	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.