14UM

Crystal Structure of FtsZ with F2146-0687

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.968, 88.968, 180.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.524 - 1.948
Rwork	0.181
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.763
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.484	1.982
High resolution limit [Å]	1.948	1.948
Rmerge	0.157	2.789
Rmeas	0.164	2.899
Rpim	0.045	0.790
Total number of observations	784531	39708
Number of reflections	58766	2957
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	13.4	13.4
CC(1/2)	0.999	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-128	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.