14UL

Crystal Structure of FtsZ with F0537-0331

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.592, 88.592, 178.099
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.335 - 1.972
Rwork	0.180
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.607
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.296	2.006
High resolution limit [Å]	1.972	1.972
Rmerge	0.207	2.566
Rmeas	0.215	2.668
Rpim	0.059	0.731
Total number of observations	739989	36959
Number of reflections	55533	2774
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	13.3	13.3
CC(1/2)	0.998	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-124	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.