14UK

Crystal Structure of FtsZ with F2199-0022

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.707, 88.707, 179.064
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.393 - 2.041
Rwork	0.181
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.777
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.354	2.076
High resolution limit [Å]	2.041	2.041
Rmerge	0.191	2.707
Rmeas	0.198	2.822
Rpim	0.054	0.787
Total number of observations	674584	32626
Number of reflections	50523	2542
<I/σ(I)>	10
Completeness [%]	100.0
Redundancy	13.4	12.8
CC(1/2)	0.998	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-123	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.