14UJ

Crystal Structure of FtsZ with F6608-1120

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.593, 88.593, 179.129
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.038 - 2.103
Rwork	0.193
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.627
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.001	2.139
High resolution limit [Å]	2.103	2.103
Rmerge	0.260	1.950
Rmeas	0.270	2.035
Rpim	0.074	0.578
Total number of observations	616491	28065
Number of reflections	46094	2274
<I/σ(I)>	7.5
Completeness [%]	100.0
Redundancy	13.4	12.3
CC(1/2)	0.997	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-122	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.