14UI

Crystal Structure of FtsZ with Z50145861

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.224, 88.224, 177.858
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.151 - 2.481
Rwork	0.166
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.571
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.112	2.524
High resolution limit [Å]	2.481	2.481
Rmerge	0.257	2.029
Rmeas	0.268	2.120
Rpim	0.074	0.596
Total number of observations	349676	15689
Number of reflections	27676	1378
<I/σ(I)>	9.1
Completeness [%]	100.0
Redundancy	12.6	11.4
CC(1/2)	0.996	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-1031	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.