14UH

Crystal Structure of FtsZ with F2167-0233

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.899, 88.899, 180.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.194 - 2.293
Rwork	0.175
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.757
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.157	2.332
High resolution limit [Å]	2.293	2.293
Rmerge	0.256	1.823
Rmeas	0.267	1.897
Rpim	0.073	0.519
Total number of observations	465159	24171
Number of reflections	35622	1831
<I/σ(I)>	7.9
Completeness [%]	98.7
Redundancy	13.1	13.2
CC(1/2)	0.995	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-100	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.