13HE
CryoEM structure of AdhE Spirosome from Clostridium thermocellum uncovered by visual proteomics.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.07 |
| Number of Particle | 94278 |
| Software | cryoSPARC,PHENIX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
