13GJ
IST1 bound to PI(4,5)P2 containing membrane
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | TFS FALCON 4i (4k x 4k),GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2 |
| Number of Particle | 3381581 |
| Software | cryoSPARC,PHENIX,SerialEM,EPU,UCSF Chimera |
| CTF correction | NONE |
