13FT

START domain of Caenorhabditis elegans StAR-related lipid transfer protein 3 (STARD3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976230
Spacegroup name	P 1 21 1
Unit cell lengths	44.774, 79.249, 72.512
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	29.670 - 2.010
R-factor	0.1821
Rwork	0.180
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.071	0.657
Rmeas	0.077	0.742
Rpim	0.029	0.337
Number of reflections	33445	2250
<I/σ(I)>	17	2.2
Completeness [%]	99.2
Redundancy	6.8
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM sodium citrate tribasic dihydrate pH 5.0, 200 mM lithium sulfate; 22% v/v PEG 200